NOTE: this post becoming too long, its content is now moved and updated on the page: PyMOL Resources PyMOL resources Summary This post provides resources on PyMOL What is PyMOL and how to get it The University of Wisconsin participates in the licensing of PyMOL Campus wide. License and software are available on the Campus …
Cryo-Electron Microscopy Cryogenic electron microscopy (cryo-EM) is a cryomicroscopy technique applied on samples cooled to cryogenic temperatures (i.e. very cold!.) For biological specimens, the structure is preserved by embedding in an environment of vitreous ice. (See more on the Wikipedia article Cryogenic electron microscopy.) The following links are videos meant to present cryo-EM visually. Unfortunately, …
Virusworld. was hosted since its inception at the Institute for Molecular Virology in 1993/1994 at the dawn of the World Wide Web and was retired on the first week of July 2022. I was the main author and curator of the web site which was started to disseminate virus depictions based on X-ray crystallography …
3D Protein structure prediction (4) This YouTube video from the European Bioinformatics Institute – EMBL-EBI webinar was recorded on 08 September 2021 This webinar will introduce AlphaFold system for prediction and interpretation of protein structures. This webinar is designed for experimental biologists who wish to understand the strengths and limitations of AlphaFold and use the …
The new ChimeraX menu "Tools > Structure Prediction > AlphaFold" provides a simple interface to run AlphaFold2 Colab that does not require the user to know how to use a Jupyter Notebook.
Single mutation to be modeled I recently had an interesting challenge, that of creating a molecular model of a protein expressed from a gene with a newly identified single amino acid mutation. I (wrongly) assumed that it would be a “simple” 3D homology modeling problem, solved by common methods. It turns out that the protein …
The Fundamentals of Biochemistry: Interactive Tutorials from Colorado State University was created by Dr. David Fahrney and edited by Ben Robinson. The 10th edition contains 10 interactive tutorials of the planned 16 chapters. Link: https://bc401.bmb.colostate.edu/ Using the javacript-based JMol web plug-in they provide easy to use, script-based interactive lessons detailing many structural aspect of biomolecules. …
I previously wrote a small booklet using PyMOL to illustrate PyMOL scripting methods and highlight some SARS-CoV-2 (COVID-19) protein structures. (See Blog titled: Covid-19 Coloring Book and PyMOL scripts book) These protein structures are explored closely in the following movie. The video below depicts COVID-19 protein structures with a scientific, biochemistry oriented voice over text. …
My little Covid-19 coloring book last month was very well received and was even reported in the CALS eNewsletter on April 17. Other people had similarly been inspired so here is a list of “More Covid-19 Coloring Books” mostly meant for younger readers (but also adults with a kid’s heart.) Some of them have a bigger …
Update 1: reported in the CALS eNewsletter on April 17 [Archived May 2, 2020] Update 2: I compiled a table with more Covid-19 coloring books from other inspired sources. Friday, April 10, 2020 (“Good Friday”) I have created a booklet titled “SARS-CoV-2 – COVID-19 | A Coloring Book” that parents who are spending a lot …