Adeno-associated virus seen along 2x icosahedral axis. Chimera image. PDB ID: 1LP3. Black background
Adeno-associated virus seen along 2x icosahedral axis. Chimera image. PDB ID: 1LP3.
Adeno-associated virus seen along 3x icosahedral axis. Chimera image. PDB ID: 1LP3.
Adeno-associated virus seen along 5x icosahedral axis. Chimera image. PDB ID: 1LP3.
Proc. Natl. Acad. Sci. U.S.A. (2002) 99 p.10405-10410
The structure of the adeno-associated virus (AAV-2) has been determined to 3-A resolution by x-ray crystallography. AAV is being developed as a vector for gene therapy to treat diseases including hemophilia, cancer, and cystic fibrosis. As in the distantly related autonomous parvoviruses, the capsid protein has a beta-barrel fold, but long loops between the beta-strands share little structural homology with other parvoviruses, leading to unique surface features. Most prominent are groups of threefold-related peaks, each an intimate association of loops from two neighboring subunits. Mutations affecting cell entry and receptor binding are clustered near the positively charged side of each peak, implicating the region in attachment to the cellular receptor, heparan sulfate proteoglycan. Amino acids involved in antibody binding are in the same general vicinity. The structure will guide rational engineering of vector capsids to tailor cellular targeting and to avoid immediate neutralization by an immune system sensitized by prior exposure to AAV.
Protein Data Bank entry: 1LP3
The Atomic Structure of Adeno-Associated Virus (AAV-2), a Vector for Human Gene Therapy
DOI: 10.2210/pdb1LP3/pdb (direct download of file)
Deposited: 2002-05-07 Released: 2002-08-07
Images and movies by Dr. Jean-Yves Sgro, UW-Madison
Software used: UCSF Chimera
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