NCBI BLAST Workshop
The NCBI workshop is designed for beginning BLAST users or people wanting a refresher on how blast works and some tips on how to run effective BLAST searches.
- Adjust search settings to avoid errors and reduce search times
- Choose the best database
- Try out the new clustered nr database
- Customize and download the search results
- Display your BLAST results in an interactive sequence viewer
BLAST: Basic Local Alignment Search Tool
BLAST is one of the most widely used bioinformatics tools in the world, most often on the NCBI web site.
BLAST was originally designed to use a nucleotide or protein sequence to search and quickly find similar sequences. BLAST is now often used for identification of: the gene name and/or source organism of the sequence, related sequences from other organisms (homologs), as well as the location of a sequence within a larger reference sequence, such as a chromosome or genome.
Basic Local Alignment Search Tool (BLAST) (Lobo, I. (2008) Basic Local Alignment Search Tool (BLAST). Nature Education 1(1):215)
Altschul, S. F., et al. Issues in searching molecular sequence databases. Nature Genetics 6, 119–129 (1994) doi:10.1038/ng0294-119 (link to article)